C19ORF80 ELISA Kit

Catalog No. ABIN2042857

Human C19ORF80 ELISA Kit Colorimetric assay for quantification of Human C19ORF80.

Quick Overview for C19ORF80 ELISA Kit (ABIN2042857)

Target: C19ORF80 (Chromosome 19 Open Reading Frame 80 (C19ORF80))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details C19ORF80 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Angiopoietin Like Protein 8 (ANGPTL8) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for C19ORF80

Target: C19ORF80 (Chromosome 19 Open Reading Frame 80 (C19ORF80))

Alternative Name: Angiopoietin Like Protein 8 (ANGPTL8)