Annexin a1 ELISA Kit

Catalog No. ABIN2042877

This Rabbit Annexin a1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rabbit Annexin a1.

Quick Overview for Annexin a1 ELISA Kit (ABIN2042877)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Rabbit

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Annexin a1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.131 ng/mL

Application Details

Plate: Pre-coated

Protocol: Annexin A1 (ANXA1) BioAssay-ELISA Kit (Rabbit) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A1 (ANXA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Annexin A1 (ANXA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Pathways: Hormone Transport