Anti-Heparin/Platelet Factor 4 Antibodies ELISA Kit

Catalog No. ABIN2042891

This Colorimetric ELISA kit is designed for the quantitative measurement of Human .

Quick Overview for Anti-Heparin/Platelet Factor 4 Antibodies ELISA Kit (ABIN2042891)

Target: Anti-Heparin/Platelet Factor 4 Antibodies

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.64 ng/mL

Application Details

Plate: Pre-coated

Protocol: Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details

Target: Anti-Heparin/Platelet Factor 4 Antibodies

Target Type: Antibody