MBL Ab ELISA Kit

Catalog No. ABIN2042897

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MBL Ab.

Quick Overview for MBL Ab ELISA Kit (ABIN2042897)

Target: MBL Ab (Anti-Mannose Binding Lectin Antibody (MBL Ab))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6.25-400 ng/mL

Application: ELISA

Product Details MBL Ab ELISA Kit

Minimum Detection Limit: 6.25 ng/mL

Brand: BioAssay™

Sensitivity: < 2.77 ng/mL

Application Details

Plate: Pre-coated

Protocol: Anti-Mannose Binding Lectin Antibody (Anti-MBL) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Mannose Binding Lectin Antibody (Anti-MBL) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mannose Binding Lectin Antibody (Anti-MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MBL Ab

Target: MBL Ab (Anti-Mannose Binding Lectin Antibody (MBL Ab))

Target Type: Antibody, Antibody