Anti-Myeloperoxidase Antibody (MPO pANCA) ELISA Kit

Catalog No. ABIN2042907

The Human Anti-Myeloperoxidase Antibody ELISA Kit (ABIN2042907) is a Colorimetric ELISA Kit designed to quantify Human Anti-Myeloperoxidase Antibody.

Quick Overview for Anti-Myeloperoxidase Antibody (MPO pANCA) ELISA Kit (ABIN2042907)

Target: Anti-Myeloperoxidase Antibody (MPO pANCA)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.12 ng/mL

Components: A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Assay Diluent A, 1x10 mL
F: TMB Substrate, 1x0 mL
G: Stop Solution, 1x0 mL
H: Wash Buffer, 30X, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: The Human Anti-Myeloperoxidase Antibody (Anti-MPO) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-MPO in human serum, plasma and other biological fluids.The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-MPO will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details

Target: Anti-Myeloperoxidase Antibody (MPO pANCA)

Target Type: Antibody