Anti-Proteolipid Protein 1, Myelin Antibody (PLP1 Ab) ELISA Kit
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- Target
- Anti-Proteolipid Protein 1, Myelin Antibody (PLP1 Ab)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 3.12-200 ng/mL
- Minimum Detection Limit
- 3.12 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 1.12 ng/mL
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- Plate
- Pre-coated
- Protocol
- Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Proteolipid Protein 1, Myelin Antibody (Anti-PLP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target
- Anti-Proteolipid Protein 1, Myelin Antibody (PLP1 Ab)
- Target Type
- Antibody
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