APOA5 ELISA Kit
Quick Overview for APOA5 ELISA Kit (ABIN2042923)
Target
See all APOA5 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 3.12 pg/mL
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Sensitivity
- < 1.45 pg/mL
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Apolipoprotein A-V (APOA5) ELISA Kit
APOA5 Reactivity: Human Colorimetric Competition ELISA 2.5 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitWide Range APOA5 Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 Reactivity: Human Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 Reactivity: Rat Colorimetric Competition ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-V (APOA5) ELISA KitAPOA5 Reactivity: Mouse Colorimetric Sandwich ELISA 3.125 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Apolipoprotein A5 (APOA5) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A5 (APOA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A5 (APOA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A5 (APOA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A5 (APOA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- APOA5 (Apolipoprotein A-V (APOA5))
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Alternative Name
- Apolipoprotein A5 (APOA5)
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Pathways
- Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism
Target See all APOA5 ELISA Kits
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