APOE ELISA Kit

Catalog No. ABIN2042927

This Colorimetric ELISA kit is designed for the quantitative measurement of Dog APOE.

Quick Overview for APOE ELISA Kit (ABIN2042927)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Dog

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.39-25 ng/mL

Application: ELISA

Product Details APOE ELISA Kit

Minimum Detection Limit: 0.39 ng/mL

Sensitivity: < 0.17 ng/mL

Application Details

Plate: Pre-coated

Protocol: Apolipoprotein E (APOE) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E (APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein E (APOE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Pathways: Regulation of Cell Size, Lipid Metabolism