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APOE ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Dog APOE.

Catalog No. ABIN2042927

Quick Overview for APOE ELISA Kit (ABIN2042927)

Target

See all APOE ELISA Kits

APOE (Apolipoprotein E (APOE))

Reactivity

10
6
5
4
3
3
3
2
2
2
2
1
1
1
1

Dog

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.39-25 ng/mL

Application

ELISA

Discover our top product APOE ELISA Kit

Minimum Detection Limit

0.39 ng/mL

Sensitivity

< 0.17 ng/mL
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APOE Reactivity: Rat Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Plate

Pre-coated

Protocol

Apolipoprotein E (APOE) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E (APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein E (APOE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions

For Research Use only
Storage

4 °C/-20 °C
Target See all APOE ELISA Kits

APOE (Apolipoprotein E (APOE))

Pathways

Regulation of Cell Size, Lipid Metabolism

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