Ataxin 10 ELISA Kit

Catalog No. ABIN2042951

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Ataxin 10.

Quick Overview for Ataxin 10 ELISA Kit (ABIN2042951)

Target: Ataxin 10 (ATXN10)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Ataxin 10 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ataxin 10 (ATXN10) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ataxin 10 (ATXN10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ataxin 10 (ATXN10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ataxin 10 (ATXN10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ataxin 10 (ATXN10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Ataxin 10

Target: Ataxin 10 (ATXN10)