ATG7 ELISA Kit

Catalog No. ABIN2042983

Human ATG7 ELISA Kit Colorimetric assay for quantification of Human ATG7.

Quick Overview for ATG7 ELISA Kit (ABIN2042983)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details ATG7 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.107 ng/mL

Application Details

Plate: Pre-coated

Protocol: Autophagy Related Protein 7 (ATG7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 7 (ATG7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 7 (ATG7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 7 (ATG7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Autophagy Related Protein 7 (ATG7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: Autophagy Related Protein 7 (ATG7)

Pathways: Response to Water Deprivation, Autophagy