BCL11A ELISA Kit

Catalog No. ABIN2042989

Human BCL11A ELISA Kit Colorimetric assay for quantification of Human BCL11A.

Quick Overview for BCL11A ELISA Kit (ABIN2042989)

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details BCL11A ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.109 ng/mL

Application Details

Plate: Pre-coated

Protocol: B-Cell CLL/Lymphoma 11A (Bcl11A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 11A (Bcl11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of B-Cell CLL/Lymphoma 11A (Bcl11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BCL11A

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Alternative Name: B-Cell CLL/Lymphoma 11A (Bcl11A)

Pathways: Regulation of Cell Size