BCL2L1 ELISA Kit

Catalog No. ABIN2042991

This Mouse BCL2L1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse BCL2L1.

Quick Overview for BCL2L1 ELISA Kit (ABIN2042991)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81-500 pg/mL

Application: ELISA

Product Details BCL2L1 ELISA Kit

Minimum Detection Limit: 7.81 pg/mL

Sensitivity: < 3.3 pg/mL

Application Details

Plate: Pre-coated

Protocol: B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L)

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling