BHMT ELISA Kit

Catalog No. ABIN2043009

This Human BHMT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human BHMT.

Quick Overview for BHMT ELISA Kit (ABIN2043009)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details BHMT ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.124 ng/mL

Application Details

Plate: Pre-coated

Protocol: Betaine Homocysteine Methyltransferase (BHMT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: Betaine Homocysteine Methyltransferase (BHMT)

Pathways: Methionine Biosynthetic Process