BMP8A ELISA Kit

Catalog No. ABIN2043013

Rat BMP8A ELISA Kit Colorimetric assay for quantification of Rat BMP8A.

Quick Overview for BMP8A ELISA Kit (ABIN2043013)

Target: BMP8A (Bone Morphogenetic Protein 8A (BMP8A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details BMP8A ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.116 ng/mL

Application Details

Plate: Pre-coated

Protocol: Bone Morphogenetic Protein 8A (BMP8A) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 8A (BMP8A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bone Morphogenetic Protein 8A (BMP8A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BMP8A

Target: BMP8A (Bone Morphogenetic Protein 8A (BMP8A))

Pathways: Negative Regulation of Hormone Secretion