BTK ELISA Kit

Catalog No. ABIN2043023

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat BTK.

Quick Overview for BTK ELISA Kit (ABIN2043023)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details BTK ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Bruton'S Tyrosine Kinase (Btk) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bruton'S Tyrosine Kinase (Btk). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bruton'S Tyrosine Kinase (Btk). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bruton'S Tyrosine Kinase (Btk), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bruton'S Tyrosine Kinase (Btk) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: Bruton'S Tyrosine Kinase (Btk)

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling