CNPY2/MSAP ELISA Kit
Quick Overview for CNPY2/MSAP ELISA Kit (ABIN2043063)
Target
See all CNPY2/MSAP (CNPY2) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.055 ng/mL
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Plate
- Pre-coated
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Protocol
- Canopy 2 Homolog (CNPY2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Canopy 2 Homolog (CNPY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Canopy 2 Homolog (CNPY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Canopy 2 Homolog (CNPY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Canopy 2 Homolog (CNPY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- CNPY2/MSAP (CNPY2) (Canopy 2 Homolog (CNPY2))
Target See all CNPY2/MSAP (CNPY2) ELISA Kits
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