Casein alpha S1 ELISA Kit

Catalog No. ABIN2043075

This Human Casein alpha S1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Casein alpha S1.

Quick Overview for Casein alpha S1 ELISA Kit (ABIN2043075)

Target: Casein alpha S1 (CSN1S1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details Casein alpha S1 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.55 ng/mL

Application Details

Plate: Pre-coated

Protocol: Casein Alpha (CSN1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Alpha (CSN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Alpha (CSN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Alpha (CSN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Casein Alpha (CSN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Casein alpha S1

Target: Casein alpha S1 (CSN1S1)

Alternative Name: Casein alpha (CSN1)