CD5L ELISA Kit

Catalog No. ABIN2043103

Human CD5L ELISA Kit Colorimetric assay for quantification of Human CD5L.

Quick Overview for CD5L ELISA Kit (ABIN2043103)

Target: CD5L (CD5 Molecule-Like (CD5L))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details CD5L ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Sensitivity: < 28.6 pg/mL

Application Details

Plate: Pre-coated

Protocol: CD5 Antigen Like Protein (CD5L) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD5 Antigen Like Protein (CD5L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD5 Antigen Like Protein (CD5L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD5 Antigen Like Protein (CD5L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of CD5 Antigen Like Protein (CD5L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD5L

Target: CD5L (CD5 Molecule-Like (CD5L))

Alternative Name: CD5 Antigen Like Protein (CD5L)