CERK ELISA Kit

Catalog No. ABIN2043115

Human CERK ELISA Kit Colorimetric assay for quantification of Human CERK.

Quick Overview for CERK ELISA Kit (ABIN2043115)

Target: CERK (Ceramide Kinase (CERK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details CERK ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.44 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ceramide Kinase (CERK) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceramide Kinase (CERK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CERK

Target: CERK (Ceramide Kinase (CERK))