CLIC4 ELISA Kit

Catalog No. ABIN2043137

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CLIC4.

Quick Overview for CLIC4 ELISA Kit (ABIN2043137)

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Application: ELISA

Product Details CLIC4 ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Sensitivity: < 30 pg/mL

Application Details

Plate: Pre-coated

Protocol: Chloride Intracellular Channel Protein 4 (CLIC4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chloride Intracellular Channel Protein 4 (CLIC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chloride Intracellular Channel Protein 4 (CLIC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CLIC4

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Alternative Name: Chloride Intracellular Channel Protein 4 (CLIC4)