CHRNB1 ELISA Kit

Catalog No. ABIN2043147

This Rat CHRNB1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat CHRNB1.

Quick Overview for CHRNB1 ELISA Kit (ABIN2043147)

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Application: ELISA

Product Details CHRNB1 ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Sensitivity: < 31 pg/mL

Application Details

Plate: Pre-coated

Protocol: Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CHRNB1

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Alternative Name: Cholinergic Receptor, Nicotinic, beta 1 (CHRNb1)

Pathways: Synaptic Membrane