CGB ELISA Kit

Catalog No. ABIN2043149

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CGB.

Quick Overview for CGB ELISA Kit (ABIN2043149)

Target: CGB (Chorionic Gonadotropin, beta Polypeptide (CGB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6.25-400 ng/mL

Application: ELISA

Product Details CGB ELISA Kit

Minimum Detection Limit: 6.25 ng/mL

Sensitivity: < 2.75 ng/mL

Application Details

Plate: Pre-coated

Protocol: Chorionic Gonadotropin Beta Polypeptide (CGb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chorionic Gonadotropin Beta Polypeptide (CGb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CGB

Target: CGB (Chorionic Gonadotropin, beta Polypeptide (CGB))

Alternative Name: Chorionic Gonadotropin beta Polypeptide (CGb)