C1orf85 ELISA Kit

Catalog No. ABIN2043151

Mouse C1orf85 ELISA Kit Colorimetric assay for quantification of Mouse C1orf85.

Quick Overview for C1orf85 ELISA Kit (ABIN2043151)

Target: C1orf85 (C1ORF85) (Chromosome 1 Open Reading Frame 85 (C1ORF85))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details C1orf85 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.123 ng/mL

Application Details

Plate: Pre-coated

Protocol: Chromosome 1 Open Reading Frame 85 (C1orf85) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromosome 1 Open Reading Frame 85 (C1orf85). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromosome 1 Open Reading Frame 85 (C1orf85). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromosome 1 Open Reading Frame 85 (C1orf85), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chromosome 1 Open Reading Frame 85 (C1orf85) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for C1orf85

Target: C1orf85 (C1ORF85) (Chromosome 1 Open Reading Frame 85 (C1ORF85))