CS ELISA Kit
Quick Overview for CS ELISA Kit (ABIN2043153)
Target
See all CS ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 6.25 ng/mL
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Sensitivity
- < 2.39 ng/mL
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Citrate Synthase (CS) ELISA Kit
CS Reactivity: Human Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Citrate Synthase (CS) ELISA KitCS Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Citrate Synthase (CS) ELISA KitCS Reactivity: Rat Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Citrate Synthase (CS) ELISA KitCS Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Citrate Synthase (CS) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Citrate Synthase (CS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Citrate Synthase (CS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Citrate Synthase (CS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Citrate Synthase (CS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- CS (Citrate Synthase (CS))
Target See all CS ELISA Kits
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