Claudin 16 ELISA Kit

Catalog No. ABIN2043161

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat Claudin 16.

Quick Overview for Claudin 16 ELISA Kit (ABIN2043161)

Target: Claudin 16 (CLDN16)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details Claudin 16 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.53 ng/mL

Application Details

Plate: Pre-coated

Protocol: Claudin 16 (CLDN16) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 16 (CLDN16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 16 (CLDN16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 16 (CLDN16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Claudin 16 (CLDN16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Claudin 16

Target: Claudin 16 (CLDN16)

Pathways: Hepatitis C