CLOCK ELISA Kit

Catalog No. ABIN2043169

Human CLOCK ELISA Kit Colorimetric assay for quantification of Human CLOCK.

Quick Overview for CLOCK ELISA Kit (ABIN2043169)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CLOCK ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.109 ng/mL

Application Details

Plate: Pre-coated

Protocol: Clock Homolog (CLOCK) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clock Homolog (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Clock Homolog (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clock Homolog (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Clock Homolog (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Alternative Name: Clock Homolog (CLOCK)

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism