C3 ELISA Kit
Quick Overview for C3 ELISA Kit (ABIN2043197)
Target
See all C3 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.25 ng/mL
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Sensitivity
- < 11.2 ng/mL
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Complement Component 3 (C3) ELISA Kit
C3 Reactivity: Human Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Mouse Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Mouse Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Rat Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Pig Colorimetric Competition ELISA 0.625 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Pig Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Human Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Mouse Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
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Plate
- Pre-coated
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Protocol
- Complement Component 3 (C3) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3 (C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 3 (C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3 (C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 3 (C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- C3 (Complement Component 3 (C3))
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Pathways
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
Target See all C3 ELISA Kits
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