Complement C4d ELISA Kit

Catalog No. ABIN2043203

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Complement C4d.

Quick Overview for Complement C4d ELISA Kit (ABIN2043203)

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 9.37-600 pg/mL

Application: ELISA

Product Details Complement C4d ELISA Kit

Minimum Detection Limit: 9.37 pg/mL

Sensitivity: < 4.2 pg/mL

Application Details

Plate: Pre-coated

Protocol: Complement Component 4d (C4d) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4d (C4d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 4d (C4d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 4d (C4d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 4d (C4d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Complement C4d

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Alternative Name: Complement Component 4d (C4d)