CRH ELISA Kit

Catalog No. ABIN2043225

This Mouse CRH ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse CRH.

Quick Overview for CRH ELISA Kit (ABIN2043225)

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 12.35-1000 pg/mL

Application: ELISA

Product Details CRH ELISA Kit

Minimum Detection Limit: 12.35 pg/mL

Sensitivity: < 4.45 pg/mL

Application Details

Plate: Pre-coated

Protocol: Corticotropin Releasing Hormone (CRH) BioAssay-ELISA Kit (Mouse) employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Corticotropin Releasing Hormone (CRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Corticotropin Releasing Hormone (CRH) and unlabeled Corticotropin Releasing Hormone (CRH) (Standards or samples) with the pre-coated antibody specific for Corticotropin Releasing Hormone (CRH). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticotropin Releasing Hormone (CRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticotropin Releasing Hormone (CRH) in the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CRH

Target: CRH (Corticotropin Releasing Hormone (CRH))

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour