DEFB126 ELISA Kit

Catalog No. ABIN2043307

Human DEFB126 ELISA Kit Colorimetric assay for quantification of Human DEFB126.

Quick Overview for DEFB126 ELISA Kit (ABIN2043307)

Target: DEFB126 (Defensin, beta 126 (DEFB126))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81-500 pg/mL

Application: ELISA

Product Details DEFB126 ELISA Kit

Minimum Detection Limit: 7.81 pg/mL

Brand: BioAssay™

Sensitivity: < 3.4 pg/mL

Application Details

Plate: Pre-coated

Protocol: Defensin Beta 126 (DEFb126) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 126 (DEFb126). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 126 (DEFb126). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 126 (DEFb126), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 126 (DEFb126) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DEFB126

Target: DEFB126 (Defensin, beta 126 (DEFB126))

Alternative Name: Defensin beta 126 (DEFb126)