DEFB131 ELISA Kit

Catalog No. ABIN2043309

This Human DEFB131 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human DEFB131.

Quick Overview for DEFB131 ELISA Kit (ABIN2043309)

Target: DEFB131 (Defensin, beta 131 (DEFB131))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details DEFB131 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.6 pg/mL

Application Details

Plate: Pre-coated

Protocol: Defensin Beta 131 (DEFb131) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 131 (DEFb131). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 131 (DEFb131). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 131 (DEFb131), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 131 (DEFb131) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DEFB131

Target: DEFB131 (Defensin, beta 131 (DEFB131))

Alternative Name: Defensin beta 131 (DEFb131)