beta 2 Defensin ELISA Kit

Catalog No. ABIN2043311

This Colorimetric ELISA kit is designed for the quantitative measurement of Cow beta 2 Defensin.

Quick Overview for beta 2 Defensin ELISA Kit (ABIN2043311)

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details beta 2 Defensin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Sensitivity: < 22.6 pg/mL

Application Details

Plate: Pre-coated

Protocol: Defensin Beta 2 (DEFb2) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 2 (DEFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 2 (DEFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 2 (DEFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 2 (DEFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for beta 2 Defensin

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Alternative Name: Defensin beta 2 (DEFb2)