DSE ELISA Kit

Catalog No. ABIN2043329

This Colorimetric ELISA kit is designed for the quantitative measurement of Human DSE.

Quick Overview for DSE ELISA Kit (ABIN2043329)

Target: DSE (Dermatan Sulfate Epimerase (DSE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details DSE ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dermatan Sulfate Epimerase (DSE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatan Sulfate Epimerase (DSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatan Sulfate Epimerase (DSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatan Sulfate Epimerase (DSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dermatan Sulfate Epimerase (DSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DSE

Target: DSE (Dermatan Sulfate Epimerase (DSE))

Pathways: Glycosaminoglycan Metabolic Process