AKR1C2 ELISA Kit

Catalog No. ABIN2043343

Human AKR1C2 ELISA Kit Colorimetric assay for quantification of Human AKR1C2.

Quick Overview for AKR1C2 ELISA Kit (ABIN2043343)

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details AKR1C2 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dihydrodiol Dehydrogenase 2 (DDH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrodiol Dehydrogenase 2 (DDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dihydrodiol Dehydrogenase 2 (DDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for AKR1C2

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Alternative Name: Dihydrodiol Dehydrogenase 2 (DDH2)

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process