DPYS ELISA Kit

Catalog No. ABIN2043345

This Human DPYS ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human DPYS.

Quick Overview for DPYS ELISA Kit (ABIN2043345)

Target: DPYS (Dihydropyrimidinase (DPYS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details DPYS ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.31 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dihydropyrimidinase (DPYS) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase (DPYS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidinase (DPYS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase (DPYS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dihydropyrimidinase (DPYS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DPYS

Target: DPYS (Dihydropyrimidinase (DPYS))