DPEP2 ELISA Kit

Catalog No. ABIN2043349

Human DPEP2 ELISA Kit Colorimetric assay for quantification of Human DPEP2.

Quick Overview for DPEP2 ELISA Kit (ABIN2043349)

Target: DPEP2 (Dipeptidase 2 (DPEP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details DPEP2 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.069 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dipeptidase 2 (DPEP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidase 2 (DPEP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidase 2 (DPEP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidase 2 (DPEP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dipeptidase 2 (DPEP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DPEP2

Target: DPEP2 (Dipeptidase 2 (DPEP2))