Dopamine Receptor d1 ELISA Kit

Catalog No. ABIN2043363

This Rat Dopamine Receptor d1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat Dopamine Receptor d1.

Quick Overview for Dopamine Receptor d1 ELISA Kit (ABIN2043363)

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Dopamine Receptor d1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.062 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dopamine Receptor D1 (DRD1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D1 (DRD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D1 (DRD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D1 (DRD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dopamine Receptor D1 (DRD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Dopamine Receptor d1

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Protein targeting to Nucleus, Feeding Behaviour, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity