DRD4 ELISA Kit

Catalog No. ABIN2043367

Rat DRD4 ELISA Kit Colorimetric assay for quantification of Rat DRD4.

Quick Overview for DRD4 ELISA Kit (ABIN2043367)

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details DRD4 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dopamine Receptor D4 (DRD4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D4 (DRD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D4 (DRD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D4 (DRD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dopamine Receptor D4 (DRD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DRD4

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Pathways: cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity