EGFL7 ELISA Kit

Catalog No. ABIN2043379

Human EGFL7 ELISA Kit Colorimetric assay for quantification of Human EGFL7.

Quick Overview for EGFL7 ELISA Kit (ABIN2043379)

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details EGFL7 ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.38 ng/mL

Application Details

Plate: Pre-coated

Protocol: EGF Like Domain Protein, Multiple 7 (EGFL7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 7 (EGFL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of EGF Like Domain Protein, Multiple 7 (EGFL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for EGFL7

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Alternative Name: EGF Like Domain Protein, Multiple 7 (EGFL7)