CELA2A ELISA Kit

Catalog No. ABIN2043389

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse CELA2A.

Quick Overview for CELA2A ELISA Kit (ABIN2043389)

Target: CELA2A (Chymotrypsin-Like Elastase Family, Member 2A (CELA2A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details CELA2A ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 12.8 pg/mL

Application Details

Plate: Pre-coated

Protocol: Elastase 2A (ELA2A) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 2A (ELA2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 2A (ELA2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 2A (ELA2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Elastase 2A (ELA2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CELA2A

Target: CELA2A (Chymotrypsin-Like Elastase Family, Member 2A (CELA2A))

Alternative Name: Elastase 2A (ELA2A)