ETFB ELISA Kit

Catalog No. ABIN2043393

This Human ETFB ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human ETFB.

Quick Overview for ETFB ELISA Kit (ABIN2043393)

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details ETFB ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.112 ng/mL

Application Details

Plate: Pre-coated

Protocol: Electron Transfer Flavoprotein Beta Polypeptide (ETFb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Beta Polypeptide (ETFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Electron Transfer Flavoprotein Beta Polypeptide (ETFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ETFB

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Alternative Name: Electron Transfer Flavoprotein beta Polypeptide (ETFb)