EVL ELISA Kit

Catalog No. ABIN2043399

This Human EVL ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human EVL.

Quick Overview for EVL ELISA Kit (ABIN2043399)

Target: EVL (Ena-Vasodilator Stimulated Phosphoprotein (EVL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details EVL ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Enah/Vasp Like Protein (EVL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enah/Vasp Like Protein (EVL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enah/Vasp Like Protein (EVL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enah/Vasp Like Protein (EVL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Enah/Vasp Like Protein (EVL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for EVL

Target: EVL (Ena-Vasodilator Stimulated Phosphoprotein (EVL))

Alternative Name: Enah/Vasp Like Protein (EVL)