ESRRA ELISA Kit

Catalog No. ABIN2043417

This Rat ESRRA ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat ESRRA.

Quick Overview for ESRRA ELISA Kit (ABIN2043417)

Target: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details ESRRA ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Estrogen Related Receptor Alpha (ERRa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Related Receptor Alpha (ERRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Related Receptor Alpha (ERRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Related Receptor Alpha (ERRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Estrogen Related Receptor Alpha (ERRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ESRRA

Target: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Alternative Name: Estrogen Related Receptor alpha (ERRa)

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha