EIF6 ELISA Kit

Catalog No. ABIN2043419

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EIF6.

Quick Overview for EIF6 ELISA Kit (ABIN2043419)

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details EIF6 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.123 ng/mL

Application Details

Plate: Pre-coated

Protocol: Eukaryotic Translation Initiation Factor 6 (EIF6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 6 (EIF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Eukaryotic Translation Initiation Factor 6 (EIF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for EIF6

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly