FAM5C ELISA Kit

Catalog No. ABIN2043439

Human FAM5C ELISA Kit Colorimetric assay for quantification of Human FAM5C.

Quick Overview for FAM5C ELISA Kit (ABIN2043439)

Target: FAM5C (Family With Sequence Similarity 5, Member C (FAM5C))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details FAM5C ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Family With Sequence Similarity 5, Member C (FAM5C) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 5, Member C (FAM5C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 5, Member C (FAM5C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 5, Member C (FAM5C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Family With Sequence Similarity 5, Member C (FAM5C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FAM5C

Target: FAM5C (Family With Sequence Similarity 5, Member C (FAM5C))