FCGBP ELISA Kit

Catalog No. ABIN2043447

This Human FCGBP ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human FCGBP.

Quick Overview for FCGBP ELISA Kit (ABIN2043447)

Target: FCGBP (Fc Fragment of IgG Binding Protein (FCGBP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details FCGBP ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.116 ng/mL

Application Details

Plate: Pre-coated

Protocol: Fc Fragment Of IgG Binding Protein (FcgBP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fc Fragment Of IgG Binding Protein (FcgBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fc Fragment Of IgG Binding Protein (FcgBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fc Fragment Of IgG Binding Protein (FcgBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fc Fragment Of IgG Binding Protein (FcgBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FCGBP

Target: FCGBP (Fc Fragment of IgG Binding Protein (FCGBP))