FKBPL ELISA Kit

Catalog No. ABIN2043491

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse FKBPL.

Quick Overview for FKBPL ELISA Kit (ABIN2043491)

Target: FKBPL (FK506 Binding Protein Like (FKBPL))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details FKBPL ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.35 ng/mL

Application Details

Plate: Pre-coated

Protocol: FK506 Binding Protein Like Protein (FKBPL) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein Like Protein (FKBPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein Like Protein (FKBPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein Like Protein (FKBPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of FK506 Binding Protein Like Protein (FKBPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FKBPL

Target: FKBPL (FK506 Binding Protein Like (FKBPL))

Alternative Name: FK506 Binding Protein Like Protein (FKBPL)