FRZB ELISA Kit

Catalog No. ABIN2043511

Human FRZB ELISA Kit Colorimetric assay for quantification of Human FRZB.

Quick Overview for FRZB ELISA Kit (ABIN2043511)

Target: FRZB (Frizzled-Related Protein (FRZB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details FRZB ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 12.9 pg/mL

Application Details

Plate: Pre-coated

Protocol: Frizzled Related Protein (FRZB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frizzled Related Protein (FRZB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frizzled Related Protein (FRZB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frizzled Related Protein (FRZB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Frizzled Related Protein (FRZB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: Frizzled Related Protein (FRZB)

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation