GPR109B ELISA Kit

Catalog No. ABIN2043521

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GPR109B.

Quick Overview for GPR109B ELISA Kit (ABIN2043521)

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details GPR109B ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: G Protein Coupled Receptor 109B (GPR109B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 109B (GPR109B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 109B (GPR109B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 109B (GPR109B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of G Protein Coupled Receptor 109B (GPR109B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GPR109B

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Alternative Name: G-Protein Coupled Receptor 109B (GPR109B)