GPR35 ELISA Kit

Catalog No. ABIN2043523

Human GPR35 ELISA Kit Colorimetric assay for quantification of Human GPR35.

Quick Overview for GPR35 ELISA Kit (ABIN2043523)

Target: GPR35 (G Protein-Coupled Receptor 35 (GPR35))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details GPR35 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.068 ng/mL

Application Details

Plate: Pre-coated

Protocol: G Protein Coupled Receptor 35 (GPR35) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 35 (GPR35). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 35 (GPR35). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 35 (GPR35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of G Protein Coupled Receptor 35 (GPR35) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GPR35

Target: GPR35 (G Protein-Coupled Receptor 35 (GPR35))

Alternative Name: G-Protein Coupled Receptor 35 (GPR35)